The US Environmental Protection Agency (EPA) has announced it will end its reliance on and funding of toxicity tests on mammals by 2035. In a memo released today, the EPA outlined steps it will take to reduce testing on mammals by 30% by 2025 and increase funding for the development of non-animal test methods. We applaud the EPA’s vision to advance animal-free testing methods that better protect humans and the environment. We look forward to continued collaborations, which have included method development, organizing validation studies, coordinating training opportunities, co-hosting free webinars, and co-authoring publications.